Characteristics of the effect of exosomes isolated from donor plasma and placenta-derived mesenchymal stromal cell-conditioned medium on the paracrine secretion of human peripheral blood mononuclear cells

Extracellular small vesicles – exosomes, attract the attention of researchers as a promising tool for regulating intercellular communication. At same time, therapeutic effects achieved through use mesenchymal stem cells (MSCs) are traditionally considered by medicine time-tested, multi-vector strategy treatment various pathologies. In particular, in addition to application MSCs directly, it is important study products their secretion. Aim. To characteristics influence exosomes isolated from blood plasma healthy donors and conditioned medium placental stromal cell (MSC) culture on paracrine secretion peripheral mononuclear (PBMCs) patients with chronic heart failure (CHF) vitro. Materials methods. The PBMCs CHF content proteins VEGF-A, MCP-1, ICAM-1 were studied two directions: under MSC medium. Results. Incubation increased VEGF-A group 2.73 times (P ≤ 0.05), 2 0.05); but there multidirectional effect protein: was 1.8 0.05) 1.4 decreased patients; MCP-1 insignificantly 10 % reduced donor did not change significantly patients. РВМС cause significant changes PBMCs: decrease 25 %, 17 22 volunteers; these 19.7 22.0 25.0 decreased, respectively, observed incubation have provided valuable information design future studies this area biology. Conclusions. Our demonstrated vitro functional properties human PBMCs.